Pro hyper hbp8
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Pro hyper hbp8
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WebOct 26, 2011 · By comparing the amino acid sequences of over 10 xylanases of family 11 from fungi, we designed two degenerate primers-Fungi1 and Fungi2 (Table 1) to amplify the partial sequence of the xylanase gene based on the HB186 genomic DNA. PCR was performed as follows: 94°C, 5 min; 30 cycles of 94°C, 30 s, 55°C, 30 s and 72°C, 50 s; and …
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WebApr 1, 2024 · Fusion proteins composed of a xylanase from Bacillus sp. HBP8 (xylHB) and double tags (MBP/Nus and 6×His, with an HRV 3C protease recognition site between them) were applied as substrates. To perform the cleavage and purification, strains expressing HRV 3C protease and the substrates were mixed before (co-fermentation method) or after …
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