Glutamine synthetase gene expression system
WebApr 11, 2024 · Here, a reporter system for rapidly investigating and optimizing reaction environments for NRPS CFE is described. This strategy is demonstrated in E. coli lysate reactions using blue pigment synthetase A (BpsA), a model NRPS, carrying a C-terminal tetracysteine (TC) tag which forms a fluorescent complex with the biarsenical dye, … WebApr 17, 2007 · Function. Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine ( By similarity ). Its role depends on tissue localization: in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the …
Glutamine synthetase gene expression system
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WebJan 1, 2010 · Cell Xpress™ Technology Facilitates High-Producing Chinese Hamster Ovary Cell Line Generation Using Glutamine Synthetase Gene Expression System January 2010 DOI: 10.1007/978-90-481-3419-9_9 WebNov 14, 2024 · To offer a bioproduction system with low expression of heterologous proteins, we explored the use of human glutamine synthetase (GLUL) gene as a selection marker for the development of producer ...
WebOf CHO cell expression systems, the dihydrofolate reductase (DHFR) and the glutamine synthetase (GS) selection systems are the most commonly used. Citation 3 In cell lines deficient in the DHFR gene, e.g., CHO-DG44 cells, selection is performed in the medium without hypoxanthine and thymidine.
WebMar 21, 2024 · Entrez Gene Summary for GLUL Gene. The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine … WebПеревод контекст "expression system" c английский на русский от Reverso Context: In some embodiments, the anti-CD40 antibody, such as CHIR-12.12, can be produced in …
WebNov 14, 2024 · To offer a bioproduction system with low expression of heterologous proteins, we explored the use of human glutamine synthetase (GLUL) gene as a selection marker for the development of producer ...
WebDec 13, 2024 · By coupling the expression of the recombinant human erythropoietin with glutamine synthetase in a bicistronic vector, erythropoietin expression was significantly improved after the sequential increase of methionine sulfoximine, a GS inhibitor (Chin et al., 2024). Application of gene amplification technologies could expand the use of human cell ... otmar wasmerWebPreviously, it was inferred that a high glutamine synthetase (GS) activity in human embryonic kidney (HEK) 293E cells results in elevated resistance to methionine sulfoximine (MSX) and consequently hampers GS-mediated gene amplification and selection by MSX. To overcome this MSX resistance in HEK293 … otmar witteWebApr 11, 2024 · Here, a reporter system for rapidly investigating and optimizing reaction environments for NRPS CFE is described. This strategy is demonstrated in E. coli lysate … otmaryWebSep 24, 2013 · The glutamine synthetase-based protein expression system is widely used in industry and academia for producing recombinant proteins but relies on the … otmar witzgallWebIn cells derived from several types of cancer, a transcriptional program drives high consumption of glutamine (Gln), which is used for anaplerosis, leading to a metabolic addiction for the amino acid. Low or absent expression of Glutamine Synthetase (GS), the only enzyme that catalyzes de novo Gln synthesis, has been considered a marker of Gln … otmar wassermannWebThe fields of study he is best known for: Gene. Enzyme. DNA. Francisco M. Cánovas mainly investigates Biochemistry, Glutamine synthetase, Botany, Chloroplast and Enzyme. His … otmar wehrleGS is subject to reversible covalent modification. Tyr of all 12 subunits can undergo adenylylation or deadenylylation by adenylyl transferase (AT), a bifunctional regulatory enzyme. Adenylylation is a post-translational modification involving the covalent attachment of AMP to a protein side chain. Each adenylylation requires an ATP and complete inhibition of GS requires 12 ATP. … rocks and waves